Skripsi kedokteran gigi yang berjudul ” APLIKASI METODE EKSTRAKSI ORGANIK PADA PEMERIKSAAN DNA BITEMARK DI BIDANG KEDOKTERAN GIGI FORENSIK Repository c” ini bisa Kamu unduh disini, dan masih banyak skripsi ter-baru lainnya.
Michael Widjaja, 020911102
APLIKASI METODE EKSTRAKSI ORGANIK PADA PEMERIKSAAN DNA BITEMARK DI BIDANG KEDOKTERAN GIGI FORENSIK.
Skripsi thesis, UNIVERSITAS AIRLANGGA.
Background: Forensic case typically encountered bitemark proof that was discovered on the sufferer’s or perpetrator’s physique. Bitemark may be in contrast with the tooth
mannequin of suspected individual bodily. Nevertheless, this method might trigger subjective interpretations. Subsequently, an alternate method to determine the bitemark case was used. The organic proof from bitemark could also be taken by
irrigating the bitemark to gather the saliva and buccal epithelium deposited throughout biting in order that the DNA from the suspected individual may be recognized. Within the reality DNA materials that has been discovered was very restricted and may be degraded, much less each in amount and high quality. As a consequence of that, an efficient technique to extract the DNA materials is required. Function: Proving the success of DNA extraction with natural technique on bitemark samples. Methodology: Blood and bitemark samples had been taken from 5 individuals, every pattern was exctracted with natural technique. Then, the pattern portions can be measured with UV-Spectrophotometer. DNA samples
had been amplified with PCR which vWA and TH01 loci had been because the goal. Visualizing band appearances had been utilizing 6% polyacrylamide gel electrophoresis. Outcome: Electrophoresis visualisations present band appearances of vWA and TH01
loci from blood and bitemark samples which imply the natural technique can be utilized for bitemark. Conclusion: It was confirmed that natural extraction can be utilized to extract DNA from bitemark.
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