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Skripsi kedokteran binatang dengan judul ” REAKSI SILANG ANTIGEN Haemonchus contortus DENGAN SERUM ANTI-Moniezia expansa MENGGUNAKAN UJI WESTERN BLOT Repository c” berikut bisa Kamu unduh disini, dan masih banyak skripsi ter-baru lainnya.

ADI PRASETYO, 061311133176

(2017)

REAKSI SILANG ANTIGEN Haemonchus contortus DENGAN SERUM ANTI-Moniezia expansa MENGGUNAKAN UJI WESTERN BLOT.

Skripsi thesis, Universitas Airlangga.

Summary

The aim of this analysis is to detect the precise protein of Haemonchus contortus which may bind anti-Moniezia expansa sera with Western blot check. Haemonchus contortus was collected from abomasum cattle. To homogenize Entire Worm Extract (WWE), mortar and stamfer are used, then add 2 ml of PBS, after that centrifuge 4000 rpm whereas 15 minutes, initiation and booster have been carried out to get polyclonal antibody from mice. Oblique ELISA used to measure sera antibody ranges. Identification of protein profile by discontinuous
system of SDS-PAGE method with Comasie Sensible Blue staining. Characterization of particular protein by Western blot check with Western Blue staining used anti-Moniezia expansa sera. The consequence confirmed the existence of cross- response between WWE Haemonchus contortus antigen with anti-Moniezia expansa sera in a protein with a molecular weight of 141, 115, 80, 56, 44, 39, 33, 28, 23, 21, 19 and 12 kDa.

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