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YUMNI KHAIRINA GHASSANI, 060810248
THE APPLICATION OF DNA BARCODING USING CYTOCHROME C OXIDASE I FOR SUMATRAN ELEPHANT (Elephas maximus Sumatrensis).
Skripsi thesis, UNIVERSITAS AIRLANGGA.
This examine was carried out to develop the molecular genetics identification for Sumatran elephant speciation utilizing mtDNA marker and to do preliminary examine for inhabitants genetic in captivity. This examine was utilizing 11 genomic DNA extracted from entire blood samples. On this examine, the conserved of COI and cyt b gene because the molecular genetics marker had been used. COI is likely one of the conserved genes of mtDNA which has very sturdy common primers for speciation. The 710-bp fragment of COI gene was amplified utilizing the COI common primers pair. All of samples confirmed no sequence divergence in comparison with the Thai elephant as reference sequence. The Sumatran elephants are clustered along with the Thai elephant in COI gene phylogenetic tree. Cyt b gene is one other conserved gene of mtDNA. Its low sequence variation charges at excessive taxonomic ranges in comparison with different vertebrates makes cyt b a good selection as a marker in speciation, phylogeography and forensic molecular genetics research. The 376-bp fragment of cyt b gene was amplified utilizing the cyt b common primers pair. The outcomes confirmed one SNP in place 14249 the place C is substituted by T. Cyt b sequence divergence outcomes confirmed very excessive sequence divergence between the samples to the African elephant and really low sequence divergence in comparison with the Asian elephants. All of the samples are clustered collectively within the cyt b gene phylogenetic tree. From each phylogenetic bushes, it may be concluded that COI and cyt b gene can be utilized for species identification.
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