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Tesis farmasi yang berjudul ” Efek Penghambatan Siklus Sel Dan Pemacuan Apoptosis Perpaduan Ekstrak Umbi Lapis Bawang Sabrang (Eleutherine bulbosa (Mill.) Urb.) dan Doksorubisin Pada Sel Kanker Payudara c” ini bisa Kamu download disini, dan masih banyak skripsi terupdate lainnya.

Bawang sabrang (Eleutherine bulbosa (Mill.) Urb.) telah dipakai secara turun temurun untuk berbagai macam penyakit seperti kanker payudara dan kolon, tekanan darah tinggi, diabetes mellitus, hiperkolesterol dan stroke. Riset ini dimaksudkan untuk mengetahui efek sitotoksik dari ekstrak umbi lapis bawang sabrang, indek selektivitas, efek kombinasinya dengan doksorubisin, siklus sel, apoptosis dan penekanan ekspresi siklin D1 dan Bcl-2 pada sel T47D dengan cara imunositokimia.
Pembuatan ekstrak dilakukan dengan metode maserasi bertingkat memakai pelarut berdasarkan tingkat kepolarannya, n-heksan, etilasetat dan etanol serta dilakukan skrining fitokimia dan karakterisasi terhadap simplisia dan ekstrak. Ekstrak diuji sitotoksiknya terhadap sel T47D dengan memakai metode MTT, indeks selektivitas, perpaduan ekstrak aktif dengan doksorubisin, siklus sel, apoptosis dan penekanan ekspresi siklin D1 dan Bcl-2 pada sel T47D dengan cara imunositokimia.
Hasil karakterisasi simplisia, ekstrak n-heksana bawang sabrang (ENBS), ekstrak etilasetat bawang sabrang (EEABS) dan ekstrak etanol bawang sabrang (EEBS) didapatkan kadar air berturut-turut eight,64%; four,98%; eight,27% dan 9,11%; kadar sari larut air eight,52%; 1,12%; 9,51% dan 72,19%; kadar sari larut etanol 14,05%; 2,28%; 49,26% dan 81,66%; kadar abu complete 2,89%; zero,34%; zero,43% dan 1,73% serta kadar abu yang tidak larut asam zero,59%; zero,06%; zero,07% dan zero,20%. Hasil skrining fitokimia simplisia dan ekstrak didapatkan golongan alkaloid, flavonoid, glikosida, saponin, antrakuinon glikosida, tannin dan triterpenoid/ steroid. Hasil pengujian sitotoksik larutan uji terhadap sel T47D memberikan nilai IC50 ENBS sebesar 265,023 µg/ml, EEABS 147,124 µg/ml dan EEBS three.782,29 µg/ml. Pengujian dengan sel vero menunjukkan EEABS selektif terhadap sel T47D (IS>three). Berikutnya EEABS dikombinasikan dengan doksorubisin terhadap sel T47D memberikan dampak sinergis kuat. Konsentrasi perpaduan optimal yang memberikan dampak sinergis kuat terdapat di konsentrasi EEABS – doksorubisin 36,75 µg/ml – 1,zero µg/ml (1/four IC50 – half IC50). EEABS dan kombinasinya dengan doksorubisin dilakukan uji siklus sel dengan cara flowsitometri, hasilnya menghambat siklus sel pada tahap G0-G1 dengan presentase 54,74% dikarenakan terjadi penekanan ekspresi siklin D1 sedangkan pengujian apoptosis menunjukkan mekanisme apoptosis dengan presentase 13,86% disebabkan terjadi penekananan ekspresi Bcl-2.

 

Sabrang onion bulbs (Eleutherine bulbosa (Mill.) Urb.) have been used for generations towards varied illnesses resembling breast and colon most cancers, hypertension, hyperglycemia, hypercholesterolemia and stroke. This analysis was aimed to find out the cytotoxic impact of sabrang onion bulbs extract, its selectivity index, its impact when mixed with doxorubicin, its cell cycle inhibition impact, its apoptosis triggering impact and its suppression of cyclin D1 and Bcl-2 expression in T47D cells. Extracts preparation was finished utilizing stepwise maceration methodology utilizing solvents primarily based on polarity degree, which have been n-hexane, ethylacetate and ethanol, and phytochemical screening together with extracts and simplex characterization have been additionally finished. The extracts have been examined for his or her cytoxicity towards T47D cells with MTT methodology, their selectivity index, mixture of energetic extracts with doxorubicin. The cell cycle inhibition impact and apoptosis triggering impact have been examined utilizing flowcytometry methodology, and the suppression of cyclin D1 and Bcl-2 expression was examined utilizing immunocytochemistry methodology. The results of simplex characterization, n-hexane extract of sabrang onion (NESO), ethylacetate extract of sabrang onion (EAESO) and ethanol extract of sabrang onion (EESO) have been as such : water content material eight.64%; four.98%; eight.27% and 9.11%; ranges of water-soluble extract eight.52%; 1.12%; 9,51% and 72.19%; ranges of ethanol soluble extract 14.05%; 2.28%; 49.26% and 81.66%; complete ash content material 2.89%, zero.34%, zero.43% and 1.73% and acid insoluble ash content material zero.59%; zero.06%; zero.07% and zero.20%, respectively. The phytochemical screening check outcomes indicated that each simplex and extract incorporates alkaloid, flavonoid, glycoside, saponin, anthraquinone glycoside, tannin and triterpenoids/steroids. The cytotoxic check on T47D cells confirmed the IC50 values of NESO at 265.023 ug/ml, EAESO 147.124 ug/ml and EESO 3782.29 mg/ml. Assessments utilizing vero cells confirmed that EAESO was selective on T47D cells (SI >three). Moreover, EAESO mixed with doxorubicin confirmed sturdy synergistic impact. The maksimal focus mixture that confirmed sturdy synergistic impact was discovered within the focus of EAESO-doxorubicin 36.75 ug/ml – 1.zero ug/ml (1/four IC50 – half IC50). The mixture of EAESO and doxorubicin inhibited the cell cycle at G0-G1 part with the proportion of 54.74% because of the suppression cyclin D1 expression, whereas the apoptosis check confirmed that the aforementioned mixture triggered apoptosis on the proportion of 13.86% because of the suppression of Bcl-2 expression.

 

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